An introduction to genetic engineering
Material type: TextSeries: Studies in biologyPublication details: New Delhi Foundation Books 2006Edition: 2nd edDescription: xii, 292 pages : ill ; 24 cmISBN:- 8175961015
- 660.65 NIC-I
Item type | Current library | Home library | Call number | Status | Date due | Barcode | |
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Book | Dept. of Computational Biology and Bioinformatics Processing Center | Dept. of Computational Biology and Bioinformatics | 660.65 NIC-I (Browse shelf(Opens below)) | Checked out to Vinni N G (86621606006) | 28/08/2022 | DCB485 | |
Book | Dept. of Computational Biology and Bioinformatics Processing Center | Dept. of Computational Biology and Bioinformatics | 660.65 NIC-I (Browse shelf(Opens below)) | Available | DCB995 | ||
Book | Dept. of Computational Biology and Bioinformatics Processing Center | Dept. of Computational Biology and Bioinformatics | 660.65 NIC-I (Browse shelf(Opens below)) | Available | DCB1242 |
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660.65 BRO-G Gene Cloning and DNA Analysis: An Introduction | 660.65 JOG-G .BT Gene Biotechnology | 660.65 NIC-I An introduction to genetic engineering | 660.65 NIC-I An introduction to genetic engineering | 660.65 NIC-I An introduction to genetic engineering | 660.65 NIC-I An introduction to genetic engineering | 660.65 SAN-U Understanding GENES & Genomes |
The basis of genetic engineering -- Introducing molecular biology -- The flow of genetic information -- The structure of DNA and RNA -- Gene organisation -- Gene structure in prokaryotes -- Gene structure in eukaryotes -- Gene expression -- Genes and genomes -- Genome size and complexity -- Genome organisation -- Working with nucleic acids -- Isolation of DNA and RNA -- Handling and quantification of nucleic acids -- Radiolabelling of nucleic acids -- End labelling -- Nick translation -- Labelling by primer extension -- Nucleic acid hybridisation -- Gel electrophoresis -- DNA sequencing -- Maxam--Gilbert (chemical) sequencing -- Sanger--Coulson (dideoxy or enzymatic) sequencing -- Electrophoresis and reading of sequences -- The tools of the trade -- Restriction enzymes--cutting DNA -- Type II restriction endonucleases -- Use of restriction endonucleases -- Restriction mapping -- DNA modifying enzymes -- Nucleases -- Polymerases -- Enzymes that modify the ends of DNA molecules -- DNA ligase--joining DNA molecules -- The methodology of gene manipulation -- Host cells and vectors -- Host cell types -- Prokaryotic hosts -- Eukaryotic hosts -- Plasmid vectors for use in E. coli -- What are plasmids? -- Basic cloning plasmids -- Slightly more exotic plasmid vectors -- Bacteriophage vectors for use in E. coli -- What are bacteriophages? -- Vectors based on bacteriophage [lambda] -- Vectors based on bacteriophage M13 -- Other vectors -- Hybrid plasmid/phage rectors -- Vectors for use in eukaryotic cells -- Artificial chromosomes.
However, the book is now divided into three main sections: the first introduces students to basic molecular biology, the second section explains the methods used to manipulate genes, and the third deals with modern applications of genetic
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