An introduction to genetic engineering
Desmond S.T. Nicholl
An introduction to genetic engineering - 2nd ed. - New Delhi Foundation Books 2006 - xii, 292 pages : ill ; 24 cm. - Studies in biology. .
The basis of genetic engineering -- Introducing molecular biology -- The flow of genetic information -- The structure of DNA and RNA -- Gene organisation -- Gene structure in prokaryotes -- Gene structure in eukaryotes -- Gene expression -- Genes and genomes -- Genome size and complexity -- Genome organisation -- Working with nucleic acids -- Isolation of DNA and RNA -- Handling and quantification of nucleic acids -- Radiolabelling of nucleic acids -- End labelling -- Nick translation -- Labelling by primer extension -- Nucleic acid hybridisation -- Gel electrophoresis -- DNA sequencing -- Maxam--Gilbert (chemical) sequencing -- Sanger--Coulson (dideoxy or enzymatic) sequencing -- Electrophoresis and reading of sequences -- The tools of the trade -- Restriction enzymes--cutting DNA -- Type II restriction endonucleases -- Use of restriction endonucleases -- Restriction mapping -- DNA modifying enzymes -- Nucleases -- Polymerases -- Enzymes that modify the ends of DNA molecules -- DNA ligase--joining DNA molecules -- The methodology of gene manipulation -- Host cells and vectors -- Host cell types -- Prokaryotic hosts -- Eukaryotic hosts -- Plasmid vectors for use in E. coli -- What are plasmids? -- Basic cloning plasmids -- Slightly more exotic plasmid vectors -- Bacteriophage vectors for use in E. coli -- What are bacteriophages? -- Vectors based on bacteriophage [lambda] -- Vectors based on bacteriophage M13 -- Other vectors -- Hybrid plasmid/phage rectors -- Vectors for use in eukaryotic cells -- Artificial chromosomes.
However, the book is now divided into three main sections: the first introduces students to basic molecular biology, the second section explains the methods used to manipulate genes, and the third deals with modern applications of genetic
8175961015
Genetic engineering.
660.65 NIC-I
An introduction to genetic engineering - 2nd ed. - New Delhi Foundation Books 2006 - xii, 292 pages : ill ; 24 cm. - Studies in biology. .
The basis of genetic engineering -- Introducing molecular biology -- The flow of genetic information -- The structure of DNA and RNA -- Gene organisation -- Gene structure in prokaryotes -- Gene structure in eukaryotes -- Gene expression -- Genes and genomes -- Genome size and complexity -- Genome organisation -- Working with nucleic acids -- Isolation of DNA and RNA -- Handling and quantification of nucleic acids -- Radiolabelling of nucleic acids -- End labelling -- Nick translation -- Labelling by primer extension -- Nucleic acid hybridisation -- Gel electrophoresis -- DNA sequencing -- Maxam--Gilbert (chemical) sequencing -- Sanger--Coulson (dideoxy or enzymatic) sequencing -- Electrophoresis and reading of sequences -- The tools of the trade -- Restriction enzymes--cutting DNA -- Type II restriction endonucleases -- Use of restriction endonucleases -- Restriction mapping -- DNA modifying enzymes -- Nucleases -- Polymerases -- Enzymes that modify the ends of DNA molecules -- DNA ligase--joining DNA molecules -- The methodology of gene manipulation -- Host cells and vectors -- Host cell types -- Prokaryotic hosts -- Eukaryotic hosts -- Plasmid vectors for use in E. coli -- What are plasmids? -- Basic cloning plasmids -- Slightly more exotic plasmid vectors -- Bacteriophage vectors for use in E. coli -- What are bacteriophages? -- Vectors based on bacteriophage [lambda] -- Vectors based on bacteriophage M13 -- Other vectors -- Hybrid plasmid/phage rectors -- Vectors for use in eukaryotic cells -- Artificial chromosomes.
However, the book is now divided into three main sections: the first introduces students to basic molecular biology, the second section explains the methods used to manipulate genes, and the third deals with modern applications of genetic
8175961015
Genetic engineering.
660.65 NIC-I