An introduction to genetic engineering (Record no. 293885)

MARC details
000 -LEADER
fixed length control field 02242nam a2200181Ia 4500
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 8175961015
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 660.65 NIC-I
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Desmond S.T. Nicholl
245 ## - TITLE STATEMENT
Title An introduction to genetic engineering
250 ## - EDITION STATEMENT
Edition statement 2nd ed.
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. New Delhi
Name of publisher, distributor, etc. Foundation Books
Date of publication, distribution, etc. 2006
300 ## - PHYSICAL DESCRIPTION
Extent xii, 292 pages : ill ; 24 cm.
490 ## - SERIES STATEMENT
Series statement Studies in biology.
505 ## - FORMATTED CONTENTS NOTE
Formatted contents note The basis of genetic engineering -- Introducing molecular biology -- The flow of genetic information -- The structure of DNA and RNA -- Gene organisation -- Gene structure in prokaryotes -- Gene structure in eukaryotes -- Gene expression -- Genes and genomes -- Genome size and complexity -- Genome organisation -- Working with nucleic acids -- Isolation of DNA and RNA -- Handling and quantification of nucleic acids -- Radiolabelling of nucleic acids -- End labelling -- Nick translation -- Labelling by primer extension -- Nucleic acid hybridisation -- Gel electrophoresis -- DNA sequencing -- Maxam--Gilbert (chemical) sequencing -- Sanger--Coulson (dideoxy or enzymatic) sequencing -- Electrophoresis and reading of sequences -- The tools of the trade -- Restriction enzymes--cutting DNA -- Type II restriction endonucleases -- Use of restriction endonucleases -- Restriction mapping -- DNA modifying enzymes -- Nucleases -- Polymerases -- Enzymes that modify the ends of DNA molecules -- DNA ligase--joining DNA molecules -- The methodology of gene manipulation -- Host cells and vectors -- Host cell types -- Prokaryotic hosts -- Eukaryotic hosts -- Plasmid vectors for use in E. coli -- What are plasmids? -- Basic cloning plasmids -- Slightly more exotic plasmid vectors -- Bacteriophage vectors for use in E. coli -- What are bacteriophages? -- Vectors based on bacteriophage [lambda] -- Vectors based on bacteriophage M13 -- Other vectors -- Hybrid plasmid/phage rectors -- Vectors for use in eukaryotic cells -- Artificial chromosomes.
520 ## - SUMMARY, ETC.
Summary, etc. However, the book is now divided into three main sections: the first introduces students to basic molecular biology, the second section explains the methods used to manipulate genes, and the third deals with modern applications of genetic
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Genetic engineering.
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Book
Holdings
Withdrawn status Lost status Damaged status Not for loan Home library Current library Shelving location Date acquired Source of acquisition Cost, normal purchase price Total Checkouts Full call number Barcode Checked out Date last seen Date last checked out Price effective from Koha item type
        Dept. of Computational Biology and Bioinformatics Dept. of Computational Biology and Bioinformatics Processing Center 05/01/2022 MBC-0450/07 195.00 1 660.65 NIC-I DCB485 28/08/2022 30/05/2022 30/05/2022 01/09/2015 Book
        Dept. of Computational Biology and Bioinformatics Dept. of Computational Biology and Bioinformatics Processing Center 02/12/2015       660.65 NIC-I DCB995   13/07/2018   02/12/2015 Book
        Dept. of Computational Biology and Bioinformatics Dept. of Computational Biology and Bioinformatics Processing Center 02/12/2015       660.65 NIC-I DCB1242   02/12/2015   02/12/2015 Book