Manipulating the mouse embryo : a laboratory manual /
Behringer, Richard.
Manipulating the mouse embryo : a laboratory manual / Richard Behringer, University of Texas, M.D. Anderson Cancer Center [and three others]. - Fourth edition. - xxii, 814 pages : illustrations ; 29 cm
Includes bibliographical references and index.
Genetics and embryology of the mouse: past, present, and future -- Summary of mouse development -- A mouse colony for the production of transgenic and chimeric animals -- Recovery and in vitro culture of preimplantation embryos -- Isolation, culture, and manipulation of postimplantation embryos -- Surgical procedures -- Production of transgenic mice by pronuclear microinjection -- Embryo-derived stem cell lines -- Germ line-competent stem cells derived from adult mice -- Vector designs for pluripotent stem cell-based transgenesis and genome alterations -- Introduction of foreign DNA into embryonic stem cells -- Production of chimeras -- Genotyping -- Parthenogenesis, pronuclear transfer, and mouse cloning -- Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection -- Cryopreservation, rederivation, and transport of mouse strains -- Techniques for visualizing gene products, cells, tissues, and organ systems -- Setting up a micromanipulation lab.
9781936113002 (hardback) 9781936113019 (paper)
2013023915
Mice--Embryos--Laboratory manuals.
Mice--Genetic engineering--Laboratory manuals.
Transgenic mice--Laboratory manuals.
Mice as laboratory animals.
SCIENCE / Life Sciences / Biology / Developmental Biology.
SCIENCE / Life Sciences / Biology / Molecular Biology.
MEDICAL / Embryology.
QL737.R6 / M2468 2014
616.027
Manipulating the mouse embryo : a laboratory manual / Richard Behringer, University of Texas, M.D. Anderson Cancer Center [and three others]. - Fourth edition. - xxii, 814 pages : illustrations ; 29 cm
Includes bibliographical references and index.
Genetics and embryology of the mouse: past, present, and future -- Summary of mouse development -- A mouse colony for the production of transgenic and chimeric animals -- Recovery and in vitro culture of preimplantation embryos -- Isolation, culture, and manipulation of postimplantation embryos -- Surgical procedures -- Production of transgenic mice by pronuclear microinjection -- Embryo-derived stem cell lines -- Germ line-competent stem cells derived from adult mice -- Vector designs for pluripotent stem cell-based transgenesis and genome alterations -- Introduction of foreign DNA into embryonic stem cells -- Production of chimeras -- Genotyping -- Parthenogenesis, pronuclear transfer, and mouse cloning -- Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection -- Cryopreservation, rederivation, and transport of mouse strains -- Techniques for visualizing gene products, cells, tissues, and organ systems -- Setting up a micromanipulation lab.
9781936113002 (hardback) 9781936113019 (paper)
2013023915
Mice--Embryos--Laboratory manuals.
Mice--Genetic engineering--Laboratory manuals.
Transgenic mice--Laboratory manuals.
Mice as laboratory animals.
SCIENCE / Life Sciences / Biology / Developmental Biology.
SCIENCE / Life Sciences / Biology / Molecular Biology.
MEDICAL / Embryology.
QL737.R6 / M2468 2014
616.027