Manipulating the mouse embryo : a laboratory manual / Richard Behringer, University of Texas, M.D. Anderson Cancer Center [and three others].
Material type: TextEdition: Fourth editionDescription: xxii, 814 pages : illustrations ; 29 cmISBN:- 9781936113002 (hardback)
- 9781936113019 (paper)
- 616.027 23
- QL737.R6 M2468 2014
- SCI072000 | SCI049000 | MED025000
Item type | Current library | Home library | Collection | Call number | Status | Date due | Barcode | |
---|---|---|---|---|---|---|---|---|
Reference | IUCEIB Library, University of Kerala General Stacks | IUCEIB Library, University of Kerala | Reference | 616.027 BEH.M (Browse shelf(Opens below)) | Not for loan | CEB056 |
Includes bibliographical references and index.
Genetics and embryology of the mouse: past, present, and future -- Summary of mouse development -- A mouse colony for the production of transgenic and chimeric animals -- Recovery and in vitro culture of preimplantation embryos -- Isolation, culture, and manipulation of postimplantation embryos -- Surgical procedures -- Production of transgenic mice by pronuclear microinjection -- Embryo-derived stem cell lines -- Germ line-competent stem cells derived from adult mice -- Vector designs for pluripotent stem cell-based transgenesis and genome alterations -- Introduction of foreign DNA into embryonic stem cells -- Production of chimeras -- Genotyping -- Parthenogenesis, pronuclear transfer, and mouse cloning -- Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection -- Cryopreservation, rederivation, and transport of mouse strains -- Techniques for visualizing gene products, cells, tissues, and organ systems -- Setting up a micromanipulation lab.
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