000			01672nam a2200193 4500
020			_a9781071612286
082			_aR572.8 _bYUA-D
245			_aDNA Modification Detection Methods/ _cEdited by Bi-Feng Yuan
260			_aNew York: _bHumana Press; _c2022.
300			_a194p. _bHB
504			_aIncludes Bibiliographical References and Index
505			_aUnderstanding the functional roles of DNA modifications relies on the accurate detection, quantification, and mapping of DNA modifications. Methods for deciphering DNA modifications have substantially improved over the last several years, which greatly revolutionize the field of DNA modifications. In addition to DNA cytosine methylation (5-methylcytosine, 5mC), the best-characterized epigenetic modification, many new modifications have been discovered to present in DNA in recent years. This book provides a comprehensive overview of available techniques and methods together with detailed step-by-step protocols for experimental procedures required to successfully perform analysis on various types of DNA modifications, including 5mC, 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxycytosine (5caC), 5-hydroxymethyluracil (5hmU), 5-formyluracil (5fU), N6-methyladenine (6mA), β-D-glucosyl-5-hydroxymethyluracil (base J) and 8-oxo-7,8-dihydroguanine (OG). This laboratory manual is a valuable source for biochemists and molecular biologists from different fields who wish to investigate DNA modifications.
650			_aDNA modification
650			_agene expression
650			_a DNA Sequencing
650			_aMass spectrometry
650			_aDetection Mapping
942			_cBK
999			_c670692 _d670692