000			01522nam a22002057a 4500
020			_a9789354643644
082			_a572.863 _bBRO/G
100			_aBrown,T.A
245			_aGene Cloning and DNA Analysis:An Introduction/ _cby T.A.Brown
250			_a8th ed.
260			_aNew Delhi: _bWiley India, _c2022.
300			_axx,450p.
505			_aPart I.The Basic Principles of Gene Cloning and DNA Analysis-- 1.Why Gene Cloning and DNA Analysis are Impotent-- 2.Vectors for Gene Cloning: Plasmids and Bacteriophages-- 3.Isolation and Purification of DNA from Living Cells-- 4.Manipulation of Purified DNA-- 5.DNA Amplification and Analysis Using Polymerase Chain Reaction-- 6.Introduction of DNA into Living Cells-- 7.Cloning Vectors for Prokaryotes (E.coli)-- 8.Cloning Vectors for Eukaryotes-- 9.How to obtail a Clone of a Specific Gene-- Part II.The Applications of Gene Cloning and DNA Analysis in Research -- 10.Sequencing Genes and Genomes-- 11.Studying Gene Expression and Function-- 12.Studying Genomes-- 13.Studying Transcriptomes and Proteomes-- Part III.The Applications of Gene Cloning and DNA Analysis in Biotechnology-- 14.Production of Protein from Cloned Genes-- 15.Use of Recombinant DNA Technology in Medicine-- 16.Use of Recombinant DNA Technology in Agriculture-- 17.DNA Analysis in Forensic Science and Archaeology.
650			_aMolecular cloning
650			_aNucleotide sequence
650			_aDNA--Analysis
650			_aDNA
650			_aRecombinant DNA
942			_cBK
999			_c669216 _d669216