000			01512cam a22001698i 4500
020			_a9780470973752 (cloth)
082	0	0	_a570.282 _bSAN-U
100	1		_aSanderson, J. B.
245	1	0	_aUnderstanding light microscopy / _cby Jeremy Sanderson.
250			_aFirst edition.
300			_apages cm
490	1		_aRoyal Microscopical Society/John Wiley Series
504			_aIncludes bibliographical references and index.
505	0		_aOur eyes and the microscope -- Light -- Basic microscope optics -- Microscope design -- Ergonomics -- Optical aberrations of the microscope -- The microscope objective -- Condensers & eyepieces -- Illumination in the microscope -- Diffraction and image formation in microscopy -- Contrast generation & enhancement -- Reflected-light microscopy -- Polarised-light microscopy : part 1, theory -- Polarised-light microscopy : part 2, applied -- Fluorescence microscopy -- Fluorophores & fluorescent proteins -- Optical sectioning & confocal microscopy -- Operating the confocal microscope -- Light-sheet microscopy -- Bleed-through & spectral unmixing -- Deconvolution -- Multi-photon microscopy -- Total internal reflection fluorescence microscopy -- Frap and fret -- Colocalisation -- Super-resolution fluorescence microscopy -- Choosing a microscope platform and core imaging facilities -- Biological specimen preparation -- Materials specimen preparation -- Recording the image : part 1, theory -- Recording the image : part 2, applied.
650		0	_aMicroscopy.
942			_cBK
999			_c261471 _d261471