000			02270cam a2200253 a 4500
020			_a9780471746614 (cloth)
020			_a0471746614 (cloth)
082	0	0	_a572.6 _bPRO
245	0	0	_aProtein purification : _bprinciples, high resolution methods, and applications / _cedited by Jan-Christer Janson.
250			_a3rd ed.
260			_aHoboken, N.J. : _bJohn Wiley & Sons, _cc2011.
300			_axiv, 517 p. : _bill. (some col.) ;
490	1		_aWiley series in methods of biochemical analysis ;
504			_aIncludes bibliographical references and index.
505	0		_aIntroduction to protein purification / B. Ersson, L. Rydén and J.-C. Janson -- Introduction to chromatography / J.-C. Janson and J.Å. Jönsson -- Gel filtration: Size exclusion chromatography / L. Hagel -- Ion exchange chromatography / E. Karlsson and I. Hirsh -- High-resolution reversed-phase chromatography of proteins / S.W. Pettersson -- Hydrophobic interaction chromatography / K.-O. Eriksson and M. Belew -- Immobilized metal ion affinity chromatography / L. Kågedal -- Covalent chromatography / F. Batista-Viera, L. Rydén and J. Carlsson -- Affinity chromatography / F. Batista-Viera, J.-C. Janson and J. Carlsson -- Affinity ligands from chemical combinatorial libraries / E. Carredano and H. Baumann -- Affinity ligands from biological combinatorial libraries / P.-Å. Nygren -- Membrane separations / J.K. Walter ... [et al.] -- Refolding of inclusion body proteins from E. coli / Z. Su, D. Lu and Z. Liu -- Purification of PEGylated proteins / C.J. Fee and J.M. Van Alstine -- Electrophoresis in gels / R. Westermeier -- Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients / P.G. Righetti, E. Fasoli and S.C. Righetti -- Two-dimensional electrophoresis in proteomics / R. Westermeier and A. Görg -- Protein elution and blotting techniques / R. Westermeier -- Capillary electrophoretic separations / W. Thormann -- High throughput screening techniques in protein purification / K.M. Lacki and E. Brekkan.
650		0	_aProteins
650		0	_aChromatographic analysis.
650		0	_aElectrophoresis.
650	1	2	_aProteins
650	2	2	_aChromatography
650	2	2	_aElectrophoresis
700	1		_aJanson, Jan-Christer.
942			_cBK
999			_c261128 _d261128