ncRNAs in inflammatory and infectious diseases / Leon N. Schulte, Wilhelm Bertrams, Christina Stielow, and Bernd Schmeck -- p73-governed miRNA networks : translating bioinformatics approaches to therapeutic solutions for cancer metastasis / Stella Logotheti, Stephan Marquardt, and Brigitte M. Pu¨tzer -- Methods for annotation and validation of circular RNAs from RNAseq data / Disha Sharma, Paras Sehgal, Judith Hariprakash, Sridhar Sivasubbu, and Vinod Scaria -- Methods to study long noncoding RNA expression and dynamics in zebrafish using RNA sequencing / Samatha Mathew, Ambily Sivadas, Paras Sehgal, Kriti Kaushik, Shamsudheen K. Vellarikkal, Vinod Scaria, and Sridhar Sivasubbu -- Workflow development for the functional characterization of ncRNAs / Markus Wolfien, David Leon Brauer, Andrea Bagnacani, and Olaf Wolkenhauer -- ncRNA editing : functional characterization and computational resources / Giovanni Nigita, Gioacchino P. Marceca, Luisa Tomasello, Rosario Distefano, Federica Calore, Dario Veneziano, Giulia Romano, Serge Patrick Nana-Sinkam, Mario Acunzo, and Carlo M. Croce -- Computational prediction of functional microRNA-mRNA interactions / Mu¨s¸erref Duygu Sac¸ar Demirci, Malik Yousef, and Jens Allmer -- Tools for understanding miRNA-mRNA interactions for reproducible RNA analysis / Andrea Bagnacani, Markus Wolfien, and Olaf Wolkenhauer -- Computational resources for prediction and analysis of functional miRNA and their targetome / Isha Monga and Manoj Kumar -- Noncoding RNAs databases : current status and trends / Vinicius Maracaja-Coutinho, Alexandre Rossi Paschoal, Jose´ Carlos Caris-Maldonado, Pedro Vinı´cius Borges, Almir Jose´ Ferreira, and Alan Mitchell Durham -- Controllability methods for identifying associations between critical control ncRNAs and human diseases / Jose C. Nacher and Tatsuya Akutsu -- Network-based methods and other approaches for predicting lncRNA functions and disease associations / Rosario Michael Piro and Annalisa Marsico -- Integration of miRNA and mRNA expression data for understanding etiology of gynecologic cancers / Sushmita Paul -- Quantitative characteristic of ncRNA regulation in gene regulatory networks / Federico Bocci, Mohit Kumar Jolly, Herbert Levine, and Jose´ Nelson Onuchic -- Kinetic modelling of competition and depletion of shared miRNAs by competing endogenous RNAs / Araks Martirosyan, Marco Del Giudice, Chiara Enrico Bena, Andrea Pagnani, Carla Bosia, and Andrea De Martino -- Modeling ncRNA-mediated circuits in cell fate decision / Xiao-Jun Tian, Manuela Vanegas Ferro, and Hanah Goetz -- Modeling long ncRNA-mediated regulation in the mammalian cell cycle / Jomar F. Rabajante and Ricardo C. H. del Rosario.

This volume details a collection of state-of-art methods including identification of novel ncRNAs and their targets, functional annotation and disease association in different biological contexts. Chapters guide readers through an overview of disease-specific ncRNAs, computational methods and workflows for ncRNA discovery, annotation based on high-throughput sequencing data, bioinformatics tools and databases for ncRNA analyses, network-based methods, and kinetic modelling of ncRNA-mediated gene regulation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Biology of Non-Coding RNA: Methods and Protocols aims to provide a state-of-the-art collection of computational methods and approaches that will be of value to researchers interested in ncRNA field.