Previous ed.: 2006.

Includes bibliographical references and index.

1. Approaches to cloning of pain-related ion channel genes / Armen N. Akopian -- 2. Mammalian expression systems and transfection techniques / Daunia Laurenti and Lezanne Ooi -- 3. Use of Escherichia coli for the production and purification of membrane proteins / Vincent G.L. Postis, Andrea E. Rawlings, Amelia Lesiuk, and Stephen A. Baldwin -- 4. Transient overexpression of genes in neurons using nucleofection / Hannah M. Kirton, Louisa Pettinger, and Nikita Gamper -- 5. Viral gene delivery : optimized protocol for production of high titer lentiviral vectors / James Hewinson, Julian F.R. Paton, and Sergey Kasparov -- 6. Two-electrode voltage clamp / Bingcai Guan, Xingjuan Chen and Hailin Zhang -- 7. Conventional micropipette-based patch clamp techniques /Jonathan D. Lippiat and David C. Wrighton -- 8. Recording of ion channel activity in planar lipid bilayer experiments / Eleonora Zakharian -- 9. Recording macroscopic currents in large patches from Xenopus oocytes / Tibor Rohacs -- 10. Combined single-channel and macroscopic recording techniques to analyze gating mechanisms of the large conductance Ca²⁺ and voltage activated (BK) potassium channel / Nguyen V. Nguyen, Aleksandra Gruslova, Wojciech A. Kosiba and Bing Wang -- 11. Perforated whole-cell patch-clamp recording / John E. Linley -- 12. Piezo-electrically driven mechanical stimulation of sensory neurons / Jizhe Hao, Jérôme Ruel, Bertrand Coste, Yann Roudaut, Marcel Crest and Patrick Dlemas -- 13. Automated planar patch-clamp / Carol J. Milligan and Clemens Möller -- 14. Recording single-channel currents using "smart patch-clamp" technique / Anamika Bhargava and Julia Gorelik -- 15. Using total internal reflection fluorescence microscopy to observe ion channel trafficking and assembly / Sarah Schwarzer, Gregory I. Mashanov, Justin E. Molloy, and Andrew Tinker -- 16. Förster resonance energy transfer-based imaging at the cell surface of live cells / Sonya M. Bierbower and Mark S. Shapiro -- 17. Use of dansyl-calmodulin to study interactions with channels and other proteins / Alessandro Alaimo, Covadonga Malo, Pilar Areso, Kerman Aloria, Oscar MIllet and Alvaro Villarroel -- 18. Imaging and quantification of recycled K[subscript ATP] channels / Christopher J. Cockcroft -- 19. Generation of antibodies that are externally acting isoform-specific inhibitors of ion channels / Jacqueline Naylor and David J. Beech -- 20. Site-directed mutagenesis to study the structure-function relationships of ion channels / Wei Yang and Lin-Hua Jiang -- 21. Cysteine-based cross-linking approach to study inter-domain interactions in ion channels / Lin-Hua Jiang -- 22. Analysis of Ca²⁺⁻ binding sites in the MthK RCK domain by x-ray crystallography / Frank J. Smith and Brad S. Rothberg -- 23. Isotope labeling strategies for analysis of an ion channel cytoplasmic domain by NMR spectroscopy / Karin Abarca-Heidemann, Elke Duchardt-Ferner, Jens Woehnert and Brad S. Rothberg -- 24. Recording dendritic ion channel properties and function from cortical neurons / Mala M. Shah -- 25. M-current recording from acute DRG slices / Kirstin E. Rose, Sylvain Gigout, and Nikita Gamper -- 26. Studying ion channels in human erythrocytes by direct and indirect means / Stephan M. Huber -- 27. Recording ion channels in isolated, split-opened tubules / Elena Mironova, Vladislav Bugay, Oleh Pochynyuk, Alexander Staruschenko and James D. Stockand -- 28. Single-channel analysis of TRPC channels in the podocytes of freshly isolated glomeruli / Daria V. Ilatovskaya and Alexander Staruschenko -- 29. Ca²+ imaging as a tool to assess TRP channel function in murine distal nephrons / Mykola Mamenko, Oleg Zaika, Roger G. O'Neil and Oleh Pochynyuk -- 30. Patch-clamping Drosophila sensory neurons / Volodymyr Kucher, Benjamin A. Eaton, James D. Stockand and Nina Boiko -- 31. Production and validation of recombinant adeno-associated virus for channelrhodopsin expression in neurons / John Y. Lin -- 32. Optical control of ligand-gated ion channels / Stephanie Szobota, Catherine McKenzie, and Harald Janovjak.

Every cell in our bodies contains a great variety and number of permeability pathways for various organic and inorganic ions, water, metabolites, nutrients, and signalling molecules. Maintenance and precise control of gating within these pathways are fundamental principles of life as these underlie basic cellular functions such as communication, contractility, and metabolism. In Ion Channels: Methods and Protocols, Second Edition, experts in the field contribute chapters that focus on the strategies, approaches, methods, and protocols for studying a large family of proteins that form ionic channels in the plasma membrane and intracellular membranes of cells. Using practical examples from the cutting-edge current research, this volume will take a look back at the major methods and approaches that aided the progress toward the current understanding of ion channel function, structural design, and biological roles. The volume also aims to look forward and identify approaches that will lead us to future discoveries. Written in the highly successful Methods in Molecular Biology [trade mark] series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Ion Channels: Methods and Protocols, Second Edition will greatly assist researchers searching for specific methodology in studying ion channels on the path toward a greater understanding of these key biological features.