TY - BOOK AU - Kappelmann-Fenzl, Melanie [Ed.] TI - Next Generation Sequencing and Data Analysis SN - 9783030624897 U1 - 572.863 3 23 PY - 2021/// CY - Switzerland PB - Springer KW - Bioinformatics- Computational biology -Genetics-Sequence Alignment KW - GENERATION SEQUENCING N1 - Chapter 1. Next Generation Sequencing (NGS)- What can be sequenced? Opportunities and Perspectives of NGS Applications in Cancer Research Chapter 3. Library Construction for NGS Chapter 4. NGS Technologies Chapter 5. Computer Setup Chapter 6. Introduction to Command Line Chapter 7. NGS Data Chapter 8. Reference Genome Chapter 9. Alignment Chapter 10. Identification of Genetic Variants and de novo Mutations Based on NGS Chapter 11. Design and Analysis of RNA Sequencing Data Chapter 12. Design and Analysis of Epigenetics and ChIP-Sequencing Data APPENDIX N2 - This textbook provides step-by-step protocols and detailed explanations for RNA Sequencing, ChIP-Sequencing and Epigenetic Sequencing applications. The reader learns how to perform Next Generation Sequencing data analysis, how to interpret and visualize the data, and acquires knowledge on the statistical background of the used software tools. Written for biomedical scientists and medical students, this textbook enables the end user to perform and comprehend various Next Generation Sequencing applications and their analytics without prior understanding in bioinformatics or computer sciences ER -