Behringer, Richard. <br/><br/>
Manipulating the mouse embryo :  a laboratory manual / 
Richard Behringer, University of Texas, M.D. Anderson Cancer Center [and three others]. 
 - Fourth edition. 
 - xxii, 814 pages :  illustrations ;  29 cm 
<br/><br/>Includes bibliographical references and index. 
<br/><br/>Genetics and embryology of the mouse: past, present, and future -- Summary of mouse development -- A mouse colony for the production of transgenic and chimeric animals -- Recovery and in vitro culture of preimplantation embryos -- Isolation, culture, and manipulation of postimplantation embryos -- Surgical procedures -- Production of transgenic mice by pronuclear microinjection -- Embryo-derived stem cell lines -- Germ line-competent stem cells derived from adult mice -- Vector designs for pluripotent stem cell-based transgenesis and genome alterations -- Introduction of foreign DNA into embryonic stem cells -- Production of chimeras -- Genotyping -- Parthenogenesis, pronuclear transfer, and mouse cloning -- Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection -- Cryopreservation, rederivation, and transport of mouse strains -- Techniques for visualizing gene products, cells, tissues, and organ systems -- Setting up a micromanipulation lab. 
<br/><br/><label>ISBN: </label>9781936113002 (hardback) 9781936113019 (paper) 
<br/><br/><label>LCCN: </label>2013023915 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Mice--Embryos--Laboratory manuals.<br/>Mice--Genetic engineering--Laboratory manuals.<br/>Transgenic mice--Laboratory manuals.<br/>Mice as laboratory animals.<br/>SCIENCE / Life Sciences / Biology / Developmental Biology.<br/>SCIENCE / Life Sciences / Biology / Molecular Biology.<br/>MEDICAL / Embryology.
<br/><br/><label>LC Class. No.: </label>QL737.R6  / M2468 2014 
<br/><br/><label>Dewey Class. No.: </label>616.027