Understanding PCR
- London, United Kingdom Academic Press is an imprint of Elsevier 2016
- ix, 87 pages : illustrations (some color) ; 23 cm.
1. Things to consider before preparing to undertake your first polymerase chain reaction -- 2. Designing and ordering your polymerase chain reaction primers -- 3. Analyzing your template DNA and/or PCR product -- 4. Quantitative PCR: things to consider -- 5. Carrying out Q-PCR -- 6. Using PCR for cloning and protein expression -- 7. Polymerase chain reaction for knocking out genes.
Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips.