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Gene Cloning and DNA Analysis:An Introduction/ by T.A.Brown

By: Material type: TextTextPublication details: New Delhi: Wiley India, 2022.Edition: 8th edDescription: xx,450pISBN:
  • 9789354643644
Subject(s): DDC classification:
  • 572.863 BRO/G
Contents:
Part I.The Basic Principles of Gene Cloning and DNA Analysis-- 1.Why Gene Cloning and DNA Analysis are Impotent-- 2.Vectors for Gene Cloning: Plasmids and Bacteriophages-- 3.Isolation and Purification of DNA from Living Cells-- 4.Manipulation of Purified DNA-- 5.DNA Amplification and Analysis Using Polymerase Chain Reaction-- 6.Introduction of DNA into Living Cells-- 7.Cloning Vectors for Prokaryotes (E.coli)-- 8.Cloning Vectors for Eukaryotes-- 9.How to obtail a Clone of a Specific Gene-- Part II.The Applications of Gene Cloning and DNA Analysis in Research -- 10.Sequencing Genes and Genomes-- 11.Studying Gene Expression and Function-- 12.Studying Genomes-- 13.Studying Transcriptomes and Proteomes-- Part III.The Applications of Gene Cloning and DNA Analysis in Biotechnology-- 14.Production of Protein from Cloned Genes-- 15.Use of Recombinant DNA Technology in Medicine-- 16.Use of Recombinant DNA Technology in Agriculture-- 17.DNA Analysis in Forensic Science and Archaeology.
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Part I.The Basic Principles of Gene Cloning and DNA Analysis--
1.Why Gene Cloning and DNA Analysis are Impotent--
2.Vectors for Gene Cloning: Plasmids and Bacteriophages--
3.Isolation and Purification of DNA from Living Cells--
4.Manipulation of Purified DNA--
5.DNA Amplification and Analysis Using Polymerase Chain Reaction--
6.Introduction of DNA into Living Cells--
7.Cloning Vectors for Prokaryotes (E.coli)--
8.Cloning Vectors for Eukaryotes--
9.How to obtail a Clone of a Specific Gene--
Part II.The Applications of Gene Cloning and DNA Analysis in Research --
10.Sequencing Genes and Genomes--
11.Studying Gene Expression and Function--
12.Studying Genomes--
13.Studying Transcriptomes and Proteomes--
Part III.The Applications of Gene Cloning and DNA Analysis in Biotechnology--
14.Production of Protein from Cloned Genes--
15.Use of Recombinant DNA Technology in Medicine--
16.Use of Recombinant DNA Technology in Agriculture--
17.DNA Analysis in Forensic Science and Archaeology.

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