Calcium techniques : a laboratory manual / edited by Jan B. Parys, KU Leuven, Martin D. Bootman, The Open University, David I. Yule, University of Rochester, Geert Bultynck, KU Leuven.
Material type:![Text](/opac-tmpl/lib/famfamfam/BK.png)
- 9781621820789 (hbk.)
- 1621820785 (hbk.)
- 9781936113583 (pbk.)
- 1936113589 (pbk.)
- 572/.516 23
- QP535.C2 C35 2014
- QV 25
Item type | Current library | Home library | Call number | Status | Date due | Barcode | |
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IUCEIB Library, University of Kerala General Stacks | IUCEIB Library, University of Kerala | 572.516 PAR.C (Browse shelf(Opens below)) | Available | CEB311 |
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572.4 STE. Molecular biology of assemblies and machines / | 572.436 BAR.B Biomolecular Thermodynamics: from theory to application | 572.45 ZOG.F From biosynthesis to total synthesis : strategies and tactics for natural products / | 572.516 PAR.C Calcium techniques : a laboratory manual / | 572.56 KIM.M Marine glycobiology : principles and applications / | 572.57 GUR.L Lipids : biology and health / | 572.57 RID.B Biochemistry of lipids, lipoproteins and membranes / |
Includes bibliographical references and index.
Fluorescence -- Luminescence -- Radioactive techniques -- Electrophysiology -- Special tissues -- NAD(P)-derived messengers -- Measuring and modeling Ca2+ dynamics.
"This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"--
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