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Signal transduction immunohistochemistry : methods and protocols / edited by Alexander E. Kalyuzhny.

Contributor(s): Material type: TextTextSeries: Springer protocols | Methods in molecular biology (Clifton, N.J.) ; v. 717.Publication details: New York, NY : Humana, c2011.Description: xiii, 302 p. : ill. (some col.) ; 27 cmISBN:
  • 9781617790232 (alk. paper)
  • 1617790230 (alk. paper)
  • 9781617790249 (eISBN)
Subject(s): DDC classification:
  • 23 571.74 ALE.S
LOC classification:
  • QR183.6 .S54 2011
NLM classification:
  • QW 504.5 S578 2011
Online resources:
Contents:
Overview of the generation, validation, and application of phosphosite-specific antibodies / Kathy Brumbaugh ... [et al.] -- Selection and validation of antibodies for signal transduction immunohistochemistry / Juraj Bodo and Eric D. Hsi -- Overview of western blotting for determining antibody specificities for immunohistochemistry / Biji T. Kurien -- Optimized protocol to make phospho-specific antibodies that work / Amy J. Archuleta ... [et al.] -- Methodology and technology for stabilization of specific states of signal transduction proteins / Mats Boren -- Enhanced antigen-retrieval protocol for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues / Sergei I. Syrbu and Michael B. Cohen -- Imaging techniques in signal transduction IHC / Jerry Sedgewick -- Practical considerations of image analysis and quantification of signal transduction IHC staining / Michael Grunkin, Jakob Raundahl, and Niels T. Foged -- Flow cytometric analysis of cell signaling proteins / Maria A. Suni and Vernon C. Maino -- CytoSys : a tool for extracting cell-cycle-related expression dynamics from static data / Jayant Avva ... [et al.] -- Signaling events initiated by kappa opioid receptor activation : quantification and immunocolocalization using phospho-selective KOR, p38 MAPK, and KIR 3.1 antibodies / Julia C. Lemos, Clarisse A. Roth, and Charles Chavkin -- Immunohistochemical assessment of signal transduction and cell-cycle networks in neural tumors / Daniel Ciznadija, Afsar Barlas, and Katia Manova -- Novel multicolor immunofluorescence technique using primary antibodies raised in the same host species / Jillian Frisch ... [et al.] -- Activation and differentiation of mesenchymal stem cells / Pravin J. Mishra and Debabrata Banerjee -- Double in situ detection of sonic hedgehog mRNA and pMAPK protein in examining the cell proliferation signaling pathway in mouse embryo / Sho Fujisawa ... [et al.] -- Identifying intracellular sites of eicosanoid lipid mediator synthesis with eicosacell assays / Christianne Bandeira-Melo, Peter F. Weller, and Patricia T. Bozza -- Absorption control in immunohistochemistry using phospho-peptides immobilized on magnetic beads / Jordan Schoephoerster ... [et al.].
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Holdings
Item type Current library Home library Collection Call number Status Date due Barcode
Book Book IUCEIB Library, University of Kerala General Stacks IUCEIB Library, University of Kerala Non-fiction 571.74 ALE.S (Browse shelf(Opens below)) Available CEB184

Includes bibliographical references and index.

Overview of the generation, validation, and application of phosphosite-specific antibodies / Kathy Brumbaugh ... [et al.] -- Selection and validation of antibodies for signal transduction immunohistochemistry / Juraj Bodo and Eric D. Hsi -- Overview of western blotting for determining antibody specificities for immunohistochemistry / Biji T. Kurien -- Optimized protocol to make phospho-specific antibodies that work / Amy J. Archuleta ... [et al.] -- Methodology and technology for stabilization of specific states of signal transduction proteins / Mats Boren -- Enhanced antigen-retrieval protocol for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues / Sergei I. Syrbu and Michael B. Cohen -- Imaging techniques in signal transduction IHC / Jerry Sedgewick -- Practical considerations of image analysis and quantification of signal transduction IHC staining / Michael Grunkin, Jakob Raundahl, and Niels T. Foged -- Flow cytometric analysis of cell signaling proteins / Maria A. Suni and Vernon C. Maino -- CytoSys : a tool for extracting cell-cycle-related expression dynamics from static data / Jayant Avva ... [et al.] -- Signaling events initiated by kappa opioid receptor activation : quantification and immunocolocalization using phospho-selective KOR, p38 MAPK, and KIR 3.1 antibodies / Julia C. Lemos, Clarisse A. Roth, and Charles Chavkin -- Immunohistochemical assessment of signal transduction and cell-cycle networks in neural tumors / Daniel Ciznadija, Afsar Barlas, and Katia Manova -- Novel multicolor immunofluorescence technique using primary antibodies raised in the same host species / Jillian Frisch ... [et al.] -- Activation and differentiation of mesenchymal stem cells / Pravin J. Mishra and Debabrata Banerjee -- Double in situ detection of sonic hedgehog mRNA and pMAPK protein in examining the cell proliferation signaling pathway in mouse embryo / Sho Fujisawa ... [et al.] -- Identifying intracellular sites of eicosanoid lipid mediator synthesis with eicosacell assays / Christianne Bandeira-Melo, Peter F. Weller, and Patricia T. Bozza -- Absorption control in immunohistochemistry using phospho-peptides immobilized on magnetic beads / Jordan Schoephoerster ... [et al.].

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