Synthetic Biology: Culture and Bioethical Considerations Introduction Perceptions and Portrayals of Synthetic Biology in Popular Culture and Politics Synthetic Biology in Academic Literature Societal Risks and Responsibilities Posed by Synthetic Biology Governance of Synthetic Biology Conclusions Acknowledgements References Synthetic Biology Standards and Methods of DNA Assembly Introduction Theory and Considerations in Double-Stranded DNA Assembly Assembly Methods in Detail Distributed Construct Assembly Methods Building Combinatorial Complexity during Assembly Emerging Technologies and Conclusions Standardisation of DNA Assembly References Standardised Genetic Output Measurement Introduction Standardisation in Conventional Biology Standardisation in Synthetic Biology Adapting Current Techniques as Synthetic Biology Standards Towards Synthetic Biology Standards for Protein Measurement Conclusions Acknowledgements References ENGINEERING BIOLOGY WITH LEGACY TOOLS Bacterial Cells as Engineered Chassis Introduction Designing and Refactoring Bacterial Genes Bacterial Synthetic Gene Networks Genome-Scale Re-Engineering of Bacterial Cells Engineering Periplasmic Space Re-Imagining the Bacterial Surface and Glycocalyx References Eukaryotae Synthetica: Synthetic Biology in Yeast, Microalgae, and Mammalian Cells Introduction Remodelling Outer Cell Membranes as Engineered Chassis Surfaces Programmable Organelles Eukaryotic Genes and Their Synthetic Counterparts Networks of Synthetic Genes in Mammalian Cells Genome-Scale Engineering in Eukaryotes Synthetic Biotherapeutics References Synthetic Plants Tools and Resources Food Security and Sustainable Agriculture Plant Chassis Engineering to Improve Nutritive Value Synthetic Biology and Green Biopharms Fibre and Fuel Future of Plant Synthetic Biology References CONSTRUCTING NEW BIOLOGIES Theory and Construction of Semi-Synthetic Minimal Cells Introduction Autopoiesis and Minimal Life Semi-Synthetic Approach to the Construction of Minimal Cells Entrapment of Solutes inside Liposomes Biotechnological Perspectives of Synthetic Cells Concluding Remarks Acknowledgements References Design Tools for Synthetic Biology Introduction Mathematical Modelling in Synthetic Biology Part Registries Device Languages Modelling Languages and Tools Network Optimisation Part Engineering DNA Editing Tools CAD Tools and Workflow Systems Summary References New Genetic Codes Introduction Available Orthogonal Pairs and Their Host Systems Using UAAs to Encode Post-Translational Modifications UAAs as Synthetic Biology Tools to Detect Interactions Occurring within Cells Photo-Activatable UAAs Bioorthogonal Chemistry Orthogonal Ribosomes Systemic Optimization of New Genetic Codes References

The Synthetic Biology Handbook explains the major goals of the field of synthetic biology and presents the technical details of the latest advances made in achieving those goals. Offering a comprehensive overview of the current areas of focus in synthetic biology, this handbook: Explores the standardisation of classic molecular bioscience approaches Addresses the societal context and potential impacts of synthetic biology Discusses the use of legacy systems as tools for new product development Examines the design and construction of de novo cells and genetic codes Describes computational methods for designing genes and gene networks Thus, the Synthetic Biology Handbook provides an accurate sense of the scope of synthetic biology today. The handbook also affords readers with an opportunity to scrutinize the underlying science and decide for themselves what aspects of synthetic biology are most valuable to their research and practice