INTRODUCTION TO BIOTECHNOLOGY Biotechnology: Scope and Importance Biosafety and Good Laboratory Practices ADVANCED TECHNIQUES IN MOLECULAR BIOLOGY Techniques of Cell Fractionation and Centrifugation Chemical Synthesis of Nucleic Acids DNA Chip Technology and its Potential Applications Bioinformatics in Biotechnology WORKING WITH NUCLEIC ACIDS Isolation of Nucleic Acids Measuring Nucleic Acid Concentration and Purity Electrophoretic Techniques DNA Sequencing Genetic Maps and Marker Analysis Polymerase Chain Reaction (PCR) In Situ Hybridization RECOMBINANT DNA AND GENETIC ENGINEERING Fundamentals of Recombinant DNA Technology Enzymes in Molecular Cloning Gene Constructs and Cloning Vectors DNA Libraries Molecular Biology of Gene Transfer Systems Selection and Screening of Recombinant Molecules APPLICATIONS OF BIOTECHNOLOGY Genetic Engineering of Microorganisms Genetic Engineering of Animals Genetic Engineering in Plants WORKING WITH PROTEINS Protein Purification Techniques Protein Detection and Estimation Protein Fractionation Techniques Immunochemical Techniques BACTERIAL AND MAMMALIAN CELL CULTURE Biology of Bacteria Cultivation of Mammalian Cells In IN VITRO PLANT CELL CULTURE AND CROP IMPROVEMENT Plant Cell Culture Laboratory and Requirements Plant Culture Media, Preparation, and Culture Initiation Micropropagation Cultures of Organized Tissues Cultures of Unorganized Tissues Cryopreservation and Distribution of Clonal Material Measurement of Plant Cell Growth and Cytological Analysis Protoplast Fusion and Somaclonal Analysis Application of Plant Cell, Tissue, and Organ Culture ENVIRONMENTAL BIOTECHNOLOGY Biotechnology in Pollution Control Biodiversity and Genetic Conservation Bioenergy Fuel from Biomass Regulatory Aspects of Using Genetically-Modified Organisms Intellectual Property Rights and Socio-Legal Aspects of Biotechnology

Molecular Biotechnology: Principles and Practices is intended as a textbook aimed at providing undergraduate and postgraduate students with a strong base in this emerging and highly promising interdisciplinary science. It strikes a balance between two important aspects of the science â€" the theory of molecular biology and the experimental approach to the study of biological processes. The main feature of this book is that it covers a wide range of molecular techniques in biotechnology and is designed to be a student- and teacher-friendly textbook. Each technique is described conceptually, followed by a detailed experimental account of the steps involved. The book can also serve as reference to the interested reader who is venturing into the field of biotechnology for the first time. Special Features -Provides comprehensive and up-to-date coverage of key concepts in biotechnology - Logical format used to provide easy access to the information - Clear and well-labelled figures