Calcium techniques : a laboratory manual / edited by Jan B. Parys, KU Leuven, Martin D. Bootman, The Open University, David I. Yule, University of Rochester, Geert Bultynck, KU Leuven.

Contributor(s):

Material type: Text

TextDescription: xvi, 592 pages : illustrations (some color)ISBN:

9781621820789 (hbk.)
1621820785 (hbk.)
9781936113583 (pbk.)
1936113589 (pbk.)

Subject(s):

DDC classification:

572.516 CAL

Contents:

Fluorescence -- Luminescence -- Radioactive techniques -- Electrophysiology -- Special tissues -- NAD(P)-derived messengers -- Measuring and modeling Ca2+ dynamics.

Summary: "This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"--

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Holdings
Item type	Current library	Home library	Call number	Status	Date due	Barcode
Book	Dept. of Biochemistry Processing Center	Dept. of Biochemistry	572.516 CAL (Browse shelf(Opens below))	Available		BCH3321

Includes bibliographical references and index.

Fluorescence -- Luminescence -- Radioactive techniques -- Electrophysiology -- Special tissues -- NAD(P)-derived messengers -- Measuring and modeling Ca2+ dynamics.

"This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"--

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