Microarry Gene Expression Data Analysis (Record no. 293623)

MARC details
000 -LEADER
fixed length control field 02071nam a2200157Ia 4500
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 9781405127356
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 572.8633 CAU-M
100 ## - MAIN ENTRY--PERSONAL NAME
Personal name Helen C Causton
245 ## - TITLE STATEMENT
Title Microarry Gene Expression Data Analysis
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Malden, MA
Name of publisher, distributor, etc. Blackwell
Date of publication, distribution, etc. 2003
300 ## - PHYSICAL DESCRIPTION
Extent viii, 160 pages : illustrations (some color) ; 24 cm
505 ## - FORMATTED CONTENTS NOTE
Formatted contents note The central dogma of molecular biology -- What are microarrays and how do they work? -- Gene function and drug discovery -- Data generation, processing and analysis: an overview -- Data management -- Experimental design -- Experimental objectives and features of microarray data -- General principles of experimental design -- Reducing the number of variables -- Time courses vs. independent data points -- Replicates and repeated measurements -- Reference samples -- Exogenous ('spiked-in') controls -- Dual labelling/dye swapping -- Validation of results -- Choice and preparation of samples -- Obtaining the appropriate sample -- Strain background -- Mutants -- Reagents -- Sample and sample composition -- Small amounts of sample: RNA amplification and pooling -- Preparation of the labelled extract -- Assessing the quality of the labelled extract -- Choice and design of arrays -- Choice of array platform -- Oligonucleotides vs. PCR products -- Replicate, guide and control features -- Cross-hybridisation -- Hybridisation, scanning and quality control -- Long-term considerations -- Record keeping -- Standardisation -- Image processing, normalisation and data transformation -- Preliminary processing of the data -- Image analysis -- Measuring and reporting expression -- Saturated pixels -- The appropriate number of pixels -- Estimating background -- Reporting expression with Affymetrix GeneChips -- Expression ratios: the starting point for sample comparison -- Transformations of the expression ratio.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element DNA microarrays. Gene expression -- Research -- Methodology. Oligonucleotide Array Sequence Analysis -- methods.
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name John Quackenbush; Alvis Brazma
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Book
Holdings
Withdrawn status Lost status Damaged status Not for loan Home library Current library Shelving location Date acquired Total Checkouts Full call number Barcode Date last seen Price effective from Koha item type
        Dept. of Computational Biology and Bioinformatics Dept. of Computational Biology and Bioinformatics Processing Center 01/09/2015   572.8633 CAU-M DCB196 01/09/2015 19/07/2019 Book