Understanding light microscopy /
Sanderson, J. B.
Understanding light microscopy / by Jeremy Sanderson. - First edition. - pages cm - Royal Microscopical Society/John Wiley Series .
Includes bibliographical references and index.
Our eyes and the microscope -- Light -- Basic microscope optics -- Microscope design -- Ergonomics -- Optical aberrations of the microscope -- The microscope objective -- Condensers & eyepieces -- Illumination in the microscope -- Diffraction and image formation in microscopy -- Contrast generation & enhancement -- Reflected-light microscopy -- Polarised-light microscopy : part 1, theory -- Polarised-light microscopy : part 2, applied -- Fluorescence microscopy -- Fluorophores & fluorescent proteins -- Optical sectioning & confocal microscopy -- Operating the confocal microscope -- Light-sheet microscopy -- Bleed-through & spectral unmixing -- Deconvolution -- Multi-photon microscopy -- Total internal reflection fluorescence microscopy -- Frap and fret -- Colocalisation -- Super-resolution fluorescence microscopy -- Choosing a microscope platform and core imaging facilities -- Biological specimen preparation -- Materials specimen preparation -- Recording the image : part 1, theory -- Recording the image : part 2, applied.
9780470973752 (cloth)
Microscopy.
570.282 / SAN-U
Understanding light microscopy / by Jeremy Sanderson. - First edition. - pages cm - Royal Microscopical Society/John Wiley Series .
Includes bibliographical references and index.
Our eyes and the microscope -- Light -- Basic microscope optics -- Microscope design -- Ergonomics -- Optical aberrations of the microscope -- The microscope objective -- Condensers & eyepieces -- Illumination in the microscope -- Diffraction and image formation in microscopy -- Contrast generation & enhancement -- Reflected-light microscopy -- Polarised-light microscopy : part 1, theory -- Polarised-light microscopy : part 2, applied -- Fluorescence microscopy -- Fluorophores & fluorescent proteins -- Optical sectioning & confocal microscopy -- Operating the confocal microscope -- Light-sheet microscopy -- Bleed-through & spectral unmixing -- Deconvolution -- Multi-photon microscopy -- Total internal reflection fluorescence microscopy -- Frap and fret -- Colocalisation -- Super-resolution fluorescence microscopy -- Choosing a microscope platform and core imaging facilities -- Biological specimen preparation -- Materials specimen preparation -- Recording the image : part 1, theory -- Recording the image : part 2, applied.
9780470973752 (cloth)
Microscopy.
570.282 / SAN-U